1-Standard Total is an ELISA test for the quantitative determination of Aflatoxins B1, B2, G1, G2 in grains, nuts, spices, cereals and animal feed.
The test provides a cost-effective, and reliable solution to the analyst, including only the standard 1 (zero standard) in the kit.
The 1-Standard ELISA series is a novel ELISA system that uses a precalibrated curve to quantify mycotoxins in under 5 minutes. Unlike the typical ELISA methodology, which requires numerous standards for proper quantification, the 1-standard ELISA protocol only requires the single (analyte-free) standard.
The key advantage of the regular ELISA, the mycotoxin test kit is that slight or occasionally larger differences in the individual experiment (time delays or temperature variations) are similar for both standards and samples, minimising their impact on the results. As a result, in order to maintain the same benefit in terms of accuracy, the 1-standard ELISA series continues to use the analyte free standard.
The precalibrated standard curve has been run several times in ProGnosis Biotech facilities, and after extensive checks and validations, it is unique for each lot and can be downloaded using the Prognosis Data Reader. When analysing a single sample, the 1-Standard ELISA requires only two ELISA wells, as opposed to six (or more) with the conventional ELISA.